expression of 17 genes in clostridium thermocellum

Expression of a celE Gene from Clostridium thermocellum in

The level of expression of the Clostridium thermocellum celE gene in the asporogenous Bacillus subtilis strain 1A718 did not exceed the endogenous background level However when transformed into sporogenous strains celE-containing constructs allowed the cells to express a high level of thermostable carboxymethylcellulase (CMCase) activity which was detected

Three cellulosomal xylanase genes in Clostridium

Oct 07 2015C thermocellum possesses seven sigma/anti-sigma systems encoded by six sigI-rsgI and one sig24C-rsi24C operons allowing it to regulate the expression of selected cellulosomal genes in response to the composition of extracellular polysaccharides One of the systems is the sigI6-rsgI6 operon which encodes a 253 amino acid alternative σ-factor σ I6 and a 760 amino acid trans

Expression of adhA from different organisms in Clostridium

Background Clostridium thermocellum is a cellulolytic anaerobic thermophile that is a promising candidate for consolidated bioprocessing of lignocellulosic biomass into biofuels such as ethanol It was previously shown that expressing Thermoanaerobacterium saccharolyticum adhA in C thermocellum increases ethanol yield In this study we investigated expression of adhA genes from different

Subcloning of β‐glucanase genes from Ruminococcus albus

Genes expressing β ‐glucanase activity have been subcloned using the shuttle vector pSA3 Expression has been obtained using Clostridium thermocellum Ruminococcus albus and Butyrivibrio fibrisolvens genes in Enterococcus facaelis and by the C thermocellum gene in Bacillus subtilis Some subclones proved lethal when β ‐glucanase activity was expressed in conjunction with tetracycline

Cloning and expression of Clostridium thermocellum genes

Jun 29 1990Vol 169 No 3 1990 June 29 1990 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Pages 1055-1060 CLONING AND EXPRESSION OF CLOSTRIDIUM THERMOCELLUM GENES CODING FOR THERMOSTABLE EXOGLUCANASES (CELLOBIOHYDROLASES) IN ESCHERICHIA COLT CELLS K Tuka V V Zverlov B K Bumazkin

Cloning of Thermostable Cellulase Genes of Clostridium

Abstract Screening for the powerful cellulase genes with improved activities remains a challenge for the biorefinery research In this study five cellobiohydrolase genes and one endoglucanase gene sourced from Clostridium thermocellum DSM 1237 cbhA celK celO cel48Y cel48S and celA were cloned into a newly established tool vector pP43JM2 and

Cloning and expression of two Clostridium thermocellum

Abstract Along with the biochemical characterization of the enzymes involved in the process of cellulose degradation byClostridium thermocellum a genetic approach based on the cloning of the genes has been developed From a gene bank of C thermocellum DNA in cosmid pHC79 (1) twoE coli clones were isolated that produced endoglucanase activity as demonstrated by the ability of crude extracts

Three cellulosomal xylanase genes in Clostridium

Oct 07 2015C thermocellum possesses seven sigma/anti-sigma systems encoded by six sigI-rsgI and one sig24C-rsi24C operons allowing it to regulate the expression of selected cellulosomal genes in response to the composition of extracellular polysaccharides One of the systems is the sigI6-rsgI6 operon which encodes a 253 amino acid alternative σ-factor σ I6 and a 760 amino acid trans

Cloning and expression of two Clostridium thermocellum

Abstract Along with the biochemical characterization of the enzymes involved in the process of cellulose degradation byClostridium thermocellum a genetic approach based on the cloning of the genes has been developed From a gene bank of C thermocellum DNA in cosmid pHC79 (1) twoE coli clones were isolated that produced endoglucanase activity as demonstrated by the ability of crude extracts

Production of cellulosic ethanol in Saccharomyces

Sep 30 2009Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion based on the native enzyme coding sequence Ethanol production depends on simultaneous saccharification of cellulose to glucose and

Expression of a celE gene from Clostridium thermocellum in

Jan 01 1995The level of expression of the Clostridium thermocellum celE gene in the asporogenous Bacillus subtilis strain 1A718 did not exceed the endogenous background level However when transformed into sporogenous strains celE-containing constructs allowed the cells to express a high level of thermostable carboxymethylcellulase (CMCase) activity which was detected exclusively

Ethanol production by the hyperthermophilic archaeon

ing AdhE-encoding genes for expression in P furiosus candidate organisms were selected based on a minimum growth temperature of 60C The AdhE gene from Clostridium thermocellum which grows optimally at 60C has been heterologously expressed in a ther-mophilic bacterium producing up to 15 mM ethanol at 65C (Chung et al 2014)

Transcriptomic analysis of Clostridium thermocellum in

Fujino T Bguin P and Aubert J (1993) "Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein CipA and a protein possibly involved in attachment of the cellulosome to the cell surface " Journal of Bacteriology 175(7) 1891-1899

Expression of a Heat

Results: Heterologous expression of the Thermoanaerobacter pseudethanolicus 39E bdhA gene that encodes an aldehyde dehydrogenase in Clostridium thermocellum significantly increased resistance to furan derivatives at concentrations found in acid-pretreated biomass

Regulation of the Cellulosomal celS (cel48A) Gene of

Clostridium thermocellum produces an extracellular multienzyme complex termed cellulosome that allows efficient solubilization of crystalline cellulose One of the major enzymes in this complex is the CelS (Cel48A) exoglucanase The regulation of CelS at the protein and transcriptional levels was studied using batch and continuous cultures

Protein Engineering of Endoglucanase CelR of Clostridium

Full Length Research Article Protein Engineering of Endoglucanase CelR of Clostridium thermocellum for Enhanced Expression Hafiz Muzzammel Rehman 1 Hira Nasir 2 Adnan Iqbal 2 Syed Zawar Shah 2 * Ammara Ahad 2 Muhammad Umair Naseem 2 Muhammad Sajjad 3 Muhammad Waheed Akhtar 4 Sajjad Ahmed 4 Adv life sci vol 6 no 2 pp 81-87 February 2019

Expression of a celE Gene from Clostridium thermocellum in

The level of expression of the Clostridium thermocellum celE gene in the asporogenous Bacillus subtilis strain 1A718 did not exceed the endogenous background level However when transformed into sporogenous strains celE-containing constructs allowed the cells to express a high level of thermostable carboxymethylcellulase (CMCase) activity which was detected

Structure of the Clostridium thermocellum gene licB and

The nucleotide sequence of the Clostridium thermocellum gene licB coding for a thermoactive β‐1 3‐1 4‐glucanase has been determined The gene is located downstream but in opposite orientation to the β‐glucosidase gene bglA A coding region of 1002 bp is flanked by canonical promoter and transcription terminator sequences

Nucleotide sequence of the celG gene of Clostridium

The nucleotide sequence of the celG gene of Clostridium thermocellum encoding endoglucanase CelG was determined The open reading frame extended over 1 698 bp and encoded a 566-amino-acid polypeptide (molecular weight of 63 128) similar to the C thermocellum endoglucanase CelB (51 5% identical residues) The N terminus displayed a typical signal peptide followed by a catalytic domain

Deletion of the Cel48S cellulase from Clostridium thermocellum

Oct 12 2010Clostridium thermocellum is a thermophilic anaerobic bacterium that rapidly solubilizes cellulose with the aid of a multienzyme cellulosome complex Creation of knockout mutants for Cel48S (also known as CelS SS and S8) the most abundant cellulosome subunit was undertaken to gain insight into its role in enzymatic and microbial cellulose solubilization

Enhanced Soluble Expression of a Thermostable Cellulase

Sep 22 2011In this study the cellulase gene celD from Clostridium thermocellum was cloned into expression vectors pET-20b(+) and pHsh While high expression can be achieved by means of both these expression systems only the pHsh expression system gives soluble proteins By weakening the mRNA secondary structure and replacing the rare codons for the N-terminal amino acids of the target

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Dec 17 20101 1 Abstract 2 A microarray study of chemostat growth on insoluble cellulose or soluble cellobiose has provided 3 substantial new information on Clostridium thermocellum gene expression This is the first 4 comprehensive examination of gene expression in C thermocellum using defined growth 5 conditions Expression was detected from 2 846 of 3 189 genes and re gression analysis

Cloning of Thermostable Cellulase Genes of Clostridium

Abstract Screening for the powerful cellulase genes with improved activities remains a challenge for the biorefinery research In this study five cellobiohydrolase genes and one endoglucanase gene sourced from Clostridium thermocellum DSM 1237 cbhA celK celO cel48Y cel48S and celA were cloned into a newly established tool vector pP43JM2 and

Expression of adhA from different organisms in Clostridium

Background Clostridium thermocellum is a cellulolytic anaerobic thermophile that is a promising candidate for consolidated bioprocessing of lignocellulosic biomass into biofuels such as ethanol It was previously shown that expressing Thermoanaerobacterium saccharolyticum adhA in C thermocellum increases ethanol yield In this study we investigated expression of adhA genes from different