detection and differentiation of potato virus y strains

Detection and Molecular Variation of Potato virus Y CP

The simultaneous differentiation of Potato virus Y strains including the newly described strain PVYNTN-NW by multiplex PCR assay Journal of Virological Methods 2010 165: 15-20 [24]Quenouille J Vassilakos N Moury B Potato virus Y: a major crop pathogen that has provided major insights into the evolution of viral pathogenicity

Discussion paper: The naming of Potato virus Y strains

Oct 18 2007Potato virus Y (PVY) strain groups are based on host response and resistance gene interactions The strain groups PVYO PVYC and PVYN are well established for the isolates infecting potato in the field A switch in the emphasis from host response to nucleotide sequence differences in the virus genomes detection of isolates recombining sequences of different strains and the need to

Potato virus Y

Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae and one of the most important plant viruses affecting potato production PVY infection of potato plants results in a variety of symptoms depending on the viral strain The mildest of these symptoms is production loss but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD)

Optimization of a magnetic capture RT

Nov 08 2017Introduction Potato virus Y (PVY) is the type member of the genus Potyvirus family Potyviridae [] It has recently been listed among the 10 most important plant viruses in the world [] and recognized as the major virus infecting potato [] A major challenge in the control of PVY infection is the continual emergence of new recombinants derived mostly from the ordinary PVY O strain and the

Immunocapture

The multiplex format of the PCR is often used for the detection and identification of multiple virus/strain simultaneously to save time labor and cost Potato virus Y (PVY) is one of the most economically important viruses infecting potato worldwide PVY exists as a complex of at least nine strains and many more unclassified recombinants that

Detection and genome characterization of Potato virus Y

Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L ) in La Union (Antioquia Colombia) Deteccin y caracterizacin del genoma de aislamientos de Potato virus Y infectando papa (Solanum tuberosum L ) en La Unin (Antioquia Colombia) Daniel Muoz E 1 Pablo Gutirrez S 1 and Mauricio

Detection and molecular characterization of Slovak tomato

virus database 322 764 were mapped Within the analysed reads a single viral species Potato virus Y was identified In total 272 063 reads (96 03% of all reads identified within the virus database) were mapped to GenBank complete genome sequence of Potato virus Y (NC_001616 1) with 93% identity

Immunocapture

The multiplex format of the PCR is often used for the detection and identification of multiple virus/strain simultaneously to save time labor and cost Potato virus Y (PVY) is one of the most economically important viruses infecting potato worldwide PVY exists as a complex of at least nine strains and many more unclassified recombinants that

Production of monoclonal antibodies against synthetic

Antibodies were prepared against two synthetic peptides P19 and P11 derived from the coat protein N‐terminal region of two pepper isolates of Potato virus Y from Tunisia (PVY‐P21 and PVY‐P2 respectively) The peptides were selected by comparing the predicted amino acid sequences of three pepper and four potato PVY isolates on the basis of their polymorphism and hydrophilicity

Production of monoclonal antibodies to potato virus Y NTN

Xianzhou Nie Rudra P Singh A new approach for the simultaneous differentiation of biological and geographical strains of Potato virus Y by uniplex and multiplex RT-PCR Journal of Virological Methods 10 1016/S0166-0934(02)00037-X 104 1 (41-54) (2002)

Biological Serological and Molecular Characterization of

A Potato virus Y potyvirus (PVY) strain was isolated from naturally infected potato (IC-RT-PCR) for differentiation of PVY strain using six specific primers pairs indicated two expected bands sizes with the understudy isolate (TBIA) is an easy and simple technique for detection of plant viruses in plant tissues directly as the sample

Detection and Differentiation of Potato Virus Y Strains by

A novel simple fast qualitative method for detection and differentiation of PVY strains in potatoes by the LightCycler technology is described Fluorescent-labeled probe designed to contain variable degree of homology with a 'target sequence' of several known reference PVY strains was annealed to PCR products of these viruses followed by a graded melting analysis

Detection and Differentiation of Potato Virus Y Strains by

Aug 08 2016A novel simple fast qualitative method for detection and differentiation of PVY strains in potatoes by the LightCycler technology is described Fluorescent-labeled probe designed to contain variable degree of homology with a 'target sequence' of several known reference PVY strains was annealed to PCR products of these viruses followed by a

Development and Evaluation of Improved Diagnostic

Identification of Potato virus Y strains associated with tuber damage during a recent virus outbreak in potato in Idaho Plant Disease 92:1371-1371 Whitworth J L S M Gray A V Karasev and J H Lorenzen 2009 Tuber symptoms are induced in potato by a range of Potato virus Y strains Phytopathology 99:S141-S141

Production and application of monoclonal antibodies

Of the MoAbs produced to the 2 strains of potato Y potyvirus PVYO-11G03 12F05 12H01 12H05 22D01 41G07 and 42A08 were specific to PVY-0 PVYT-4E7 and 6C11 to PVY-T and PVY0-11C01 12H03 and 22A02 were common to both strains in indirect double antibody sandwich ELISA using polyclonal antibodies (PoAbs) for trapping antibodies In indirect double antibody sandwich ELISA

Application of New Diagnostic Reagents for Identification

Progress 10/01/12 to 09/30/13 Outputs Progress Report Objectives (from AD-416): Develop and test new diagnostic antibody and molecular reagents for detection and identification of strains of potato virus Y Approach (from AD-416): Purified nucleoproteins of newly identified strains of potato virus Y will be purified in the laboratory of the SY with the U of Idaho

Brief Review Discussion paper: The naming of Potato virus

strain concept Introduction Potato virus Y (PVY species Potato virus Y) is the type member of genus Potyvirus the largest group of plant viruses containing 128 approved and 89 tentative species [30] According to potato special-ists in most parts of the world PVY is currently Correspondence: R P Singh Potato Research Centre

Complete nucleotide sequence of a potato isolate of strain

(2007) Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers (2008) Discussion paper: The naming of Potato virus Y strains infecting potato (2002) Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses (1970)

Continuous and Emerging Challenges of Potato virus Y in

Potato virus Y (PVY) is one of the oldest known plant viruses and yet in the past 20 years it emerged in the United States as a relatively new and very serious problem in potato The virus exists as a complex of strains that induce a wide variety of foliar and tuber symptoms in potato leading to yield reduction and loss of tuber quality

CHARACTERIZATION OF POTATO VIRUS Y PVY ISOLATES

i Abstract Potato virus Y (PVY) is an economically important virus worldwide In South Africa PVY has been shown to be a major limiting factor in the production of important solanaceous crops including potato (Solanum tuberosum L ) pepper (Capsicum annuum L ) tomato (Lycopersicon esculentum Mill ) and tobacco (Nicotiana spp) The variability that PVY displays wherever the virus occurs

Identification and detection of Potato virus Y strains by

Identification and detection of Potato virus Y strains by molecular methods in tobacco fields of North Iran Fateme zinati fakhrabad 1* Asadollah ahmadikhah 2 and saeed nasrollahnejad 3 1- Former Graduate student Gorgan University of Agriculture Sciences and Natural Resources 2- Assistant Professor Department of Agriculture Biotecnology

The effects of potato virus Y

Jul 17 2017Potato virus Y (PVY) continues to be an economically important pathogen of potato (Solanum tuberosum L ) worldwide that causes significant yield losses and is detrimental to quality of tubers [1 2 3] PVY belongs to the family Potyviridae and genus Potyvirus It is a flexuous rod-shaped virus which exhibits a 9 7 kb + ss RNA genome [] The genome consists of two opening reading