development of specific marker for pcr diagnostic of

Development of SCAR marker for Specific Detection of

The specificity of the marker when tested against six isolates of species showed a Trichoderma specific band of 220 bp only in T harzanium and a specific band of 900 bp only in T viride with the optimized PCR parameters This sequence characterized amplified region (SCAR) marker

Diagnostic detection of 2019

Real-time reverse-transcription polymerase chain reaction All assays used the same conditions Primer and probe sequences as well as optimized concentrations are shown in Table 1 A 25-μl reaction was set up containing 5 μl of RNA 12 5 μl of 2 X reaction buffer provided with the Superscript III one step RT-PCR system with

Development of PCR assays diagnostic for RFLP marker

Marker technology based on the polymerase chain reaction (PCR) would facilitate the application of marker-assisted selection PCR assays have been developed that are diagnostic for RFLP alleles at two marker loci CP56 and CP113 which are closely linked in coupling to the nematode resistance alleles Gro1 on chromosome VII and H1 on chromosome

Nucleic Acid

Nucleic acid-based diagnostics detect the presence of a pathogen either by directly detecting the presence of DNA or RNA nucleic acids in the host or by first amplifying the pathogen DNA or RNA Nucleic acid-based diagnostics are a standard central laboratory technique although simplified nucleic acid-based diagnostics that may be useful in resource-poor settings are emerging

Molecular Diagnostics

PCR is now the most commonly used molecular diagnostic tool offering a very sensitive and rapid approach for the detection identification and quantification of specific DNA or RNA targets More recently real-time PCR utilizing fluorescent dye detection has streamlined the use of NAATs - improving quantification applications turn-around

DEVELOPMENT OF SCAR MARKERS AND PCR ASSAY FOR

A PCR assay based on race-specific SCAR markers for FOM race 2 identification was developed which proved specific sensitive and reliable regardless of the origin of isolates SCAR primers developed from RAPD markers were able to identify unambiguously FOM race 2 that causes symptoms similar to those elicited by the other FOM races

Development of PCR assay to identify Pseudomonas

The aim of this work was: (i) to develop a rapid polymerase chain reaction (PCR)-based species-specific protocol to identify Ps fluorescens and (ii) to distinguish the different biotypes of this species by combining molecular techniques with traditional biochemical methods 2 Materials and

Development of diagnostic SNP markers for quality

Apr 24 2020However for routine QC this marker number is still probably too high for most breeding programs Principal component analysis of the 85 markers did not show any specific grouping of markers with PC1 and PC2 only explaining 9 1% of the variation Therefore the final 30 SNP markers were selected based on genetic distance per chromosome

TipMT: Identification of PCR

Keywords: Molecular marker Specific primers PCR PCR Multiplex Web application Background Polymerase chain reaction (PCR)-based typing methods are molecular diagnostic techniques widely used in bio-logical and biomedical studies The level of discrimin-atory power of PCR-based typing depends upon the molecular marker targeted

Development of two sequence

Among the markers originated from 153 sets of MFLPs during a mapping study (Boersma et al 2005) two dominant markers designated as DAWA323 150 and DAWA468 290 were identified as candidate markers for development as sequence-specific markers tagging the le gene

Development of a SCAR Marker

May 20 2020Objective Due to the allergic nature of the pollen of Cryptomeria japonica the most important Japanese forestry conifer a pollen-free cultivar is preferred Mutant trees detected in nature have been used for the production of a pollen-free cultivar In order to reduce the time and cost needed for the production and breeding we aimed to develop simple diagnostic molecular markers for

Development of specific molecular markers to distinguish

Apr 15 2019Furthermore using ITS-100 marker and Real-Time PCR analysis allowed quantitative diagnostic of the parasite in a soil sample from infected sunflower field As expected the universal internal control primer (UCP-555) amplified a PCR product (555bp) when genomic DNA extracted from soil samples with or without broomrape tissues

Development of PCR test kits for diagnostics

Objective – development and production of domestic PCR test kits for detection and differentiation of Brucella species in Kazakhstan according to their generic and specific markers Expected results: 1 Development of primer system for detection of generic and specific genetic markers of bacteria belonging to Brucella genus 2

Molecular

Reverse transcription loop-mediated isothermal amplification (RT-LAMP): Rapid amplification of viral genomic material coupled with a color- or light-based readout and it can be performed at a single temperature unlike rRT-PCR Pros: Requires a single temperature only rapid (minutes to results) point-of-care appropriate highly sensitive and specific to defined SARS-CoV-2 sequences

Leishmania aethiopica: Development of specific and

Aug 01 2011Table 1 Primers and PCR conditions used in the study V5F/V10R primers are L aethiopica specific primers amplifying 564 bp product from cpb genes CpbF/CpbR primers amplify 735 bp product from all Leishmania species β-actinF/β-actinR primers amplify 800 bp product from human DNA sample CpbATG/cpbTAG was used in the study for amplification of cpb open reading frames

Biomarker (medicine)

In medicine a biomarker is a measurable indicator of the severity or presence of some disease state More generally a biomarker is anything that can be used as an indicator of a particular disease state or some other physiological state of an organism A biomarker can be a substance that is introduced into an organism as a means to examine organ function or other aspects of health

Quick PCR based diagnosis of typhoid using specific

Feb 06 2010A quick multiplex PCR based detection method was developed for early diagnosis of typhoid using specific genetic markers of S typhi Primers of tyv gene flag gene viaB gene and ratA gene confirmed the specificity and sensitivity of the PCR The serum samples of the suspected typhoid patients were taken directly for PCR without culturing and isolating genomic DNA Overall diagnosis

Development and utilization of diagnostic DAMD

Jul 22 2008Abstract A polymerase chain reaction (PCR) based approach involving the directed amplification of minisatellite DNA region (DAMD-PCR) was used to identify accession specific DNA markers and study genetic relationships between and within 15 accessions corresponding to 11 species in genus Capsicum A touch down PCR profile and unique chemical concentration of ingredients

TipMT: Identification of PCR

Keywords: Molecular marker Specific primers PCR PCR Multiplex Web application Background Polymerase chain reaction (PCR)-based typing methods are molecular diagnostic techniques widely used in bio-logical and biomedical studies The level of discrimin-atory power of PCR-based typing depends upon the molecular marker targeted

CiteSeerX — Development of molecular diagnostic markers

CiteSeerX - Document Details (Isaac Councill Lee Giles Pradeep Teregowda): Reporting Period: The results reported here are from work conducted fiscal year 2004 to fiscal year 2005 To aid in identifying key predators of Proconiini sharpshooter species present in California we developed and tested molecular diagnostic markers for the glassy-winged sharpshooter Homalodisca coagulata (Say) and

Development of a Non

Development of a Non-Invasive Method Multiplex Methylation Specific PCR (MMSP) for Early Diagnosis of Nasopharyngeal Carcinoma Zhe Zhang1 2 Di Sun1 2 Susanna Hilda Hutajulu3 Imran Nawaz1 Do Nguyen Van1 5 Guangwu Huang2 Sofia M Haryana3 Jaap M Middeldorp4 Ingemar Ernberg1 Li-Fu Hu1* 1Department of Microbiology Tumor and Cell Biology (MTC) Karolinska

Development of a molecular marker for specific detection

For this diagnostic purpose we have developed a reliable molecular method to detect Foc race 4 isolates in Taiwan By PCR amplification the primer set Foc-1/Foc-2 derived from the sequence of a random primer OP-A02 amplified fragment produced a 242 bp size DNA fragment which was specific

Development of a molecular marker for specific detection

For this diagnostic purpose we have developed a reliable molecular method to detect Foc race 4 isolates in Taiwan By PCR amplification the primer set Foc-1/Foc-2 derived from the sequence of a random primer OP-A02 amplified fragment produced a 242 bp size DNA fragment which was specific

Frontiers

Objective: To explore the diagnostic value of serum severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein assay in the early stages of SARS-COV-2 infection Methods: Serum N protein level in SARS-COV-2 infected patients and non-SARS-COV-2 infected population was measured by enzyme-linked immunosorbent assay (ELISA) double antibody sandwich assay

Quick PCR based diagnosis of typhoid using specific

Feb 06 2010A quick multiplex PCR based detection method was developed for early diagnosis of typhoid using specific genetic markers of S typhi Primers of tyv gene flag gene viaB gene and ratA gene confirmed the specificity and sensitivity of the PCR The serum samples of the suspected typhoid patients were taken directly for PCR without culturing and isolating genomic DNA Overall diagnosis

CiteSeerX — Development of molecular diagnostic markers

CiteSeerX - Document Details (Isaac Councill Lee Giles Pradeep Teregowda): Reporting Period: The results reported here are from work conducted fiscal year 2004 to fiscal year 2005 To aid in identifying key predators of Proconiini sharpshooter species present in California we developed and tested molecular diagnostic markers for the glassy-winged sharpshooter Homalodisca coagulata (Say) and

Development of a SCAR marker for detection of Bipolaris

Spot blotch of wheat caused by Bipolaris sorokiniana is an important disease of wheat especially in slightly warm (25 1 C) and humid weather conditions A quick and reliable PCR-based diagnostic assay has been developed to detect B sorokiniana using a pathogen-specific marker derived from genomic DNA A PCR-amplified band of 650 bp obtained in B sorokiniana isolates using universal

Development of specific molecular markers to distinguish

Apr 15 2019Furthermore using ITS-100 marker and Real-Time PCR analysis allowed quantitative diagnostic of the parasite in a soil sample from infected sunflower field As expected the universal internal control primer (UCP-555) amplified a PCR product (555bp) when genomic DNA extracted from soil samples with or without broomrape tissues